英语翻译In support of a critical role for the BRCA1 complex in c

英语翻译
In support of a critical role for the BRCA1 complex in chromosome end protection and in agreement with previous reports28,29,we show that inhibition of BRCA1,RAP80 (also known as UIMC1) or BRCC3 results in partial loss of end protection (Supplementary Fig.16).The identification of BRCC3 as a critical factor involved in TRF2-dependent telomere protection suggests that an important physiological function of the BRCA1 complex is to maintain genomic stability by aiding telomere-associated proteins in maintaining telomere integrity.
Mice and MEFs
Rosa26-CreERT2 mice (Jackson Laboratories) and mice carrying a conditional TRF2 construct1 were crossed to generate MEFs.MEFs were immortalized with pBabeSV40LT and treated 4-hydroxytamoxifen (0.6 μM) to induce Cre-mediated recombination.
Constructs,plasmids and viral infections
TRFc constructs were generated by PCR amplification using as templates pBabe-MYC-TRF1 or pBabe-MYC-TRF2 constructs (primers used are listed in Supplementary Table 2).pLDT-GFP-RNF8,pLDT-GFP-RNF168 and GFP-MDC1 were a gift from M.D.Weitzman.pOZ-FH-BRCC3 was obtained from Addgene.pcDNA-MYC-MRE11 and pcDNA-MYC-NBS1 were a gift from X.Wu.
Immunofluorescence,FISH and ChIP
Immunofluorescence,fluorescence in situ hybridization and chromatin immunoprecipitation (ChIP) experiments were performed as described previously2.

在染色体末端的保护BRCA1复杂关键作用的支持,与以前的reports28,29的协议,我们表明,抑制BRCA1,RAP80（也被称为uimc1）或brcc3结果在末端保护的部分损失（补充图16）.brcc3的识别是一个关键因素参与TRF2依赖端粒保护表明BRCA1复杂重要的生理功能是维持端粒相关蛋白的协助维持端粒完整基因组的稳定性.小鼠和mefsrosa26-creert2小鼠（杰克逊实验室）和承载条件TRF2 construct1小鼠杂交产生细胞.MEF是不死的pbabesv40lt处理与4-羟基他莫昔芬（0.μM）诱导的Cre介导的重组质粒和病毒infectionstrfc.构建,并通过模板pbabe-myc-trf1或pbabe-myc-trf2构建PCR扩增产生的（引物在补充表2列出）.pldt-gfp-rnf8,pldt-gfp-rnf168和gfp-mdc1从医学博士韦茨曼的礼物.poz-fh-brcc3获得addgene.pcdna-myc-mre11和pcdna-myc-nbs1从X.Wu的礼物.免疫荧光,鱼和chipimmunofluorescence,荧光原位杂交和染色质免疫沉淀（ChIP）实验进行了previously2.